MARK4ren interakzioak USP21 deubikuitinasaren kokapen azpizelular dinamikoa erregulatzen du

Abstract

The deubiquitinase USP21 shuttles between the nucleus and the cytoplasm and can also localize to the centrosome, but how this complex subcellular localization is modulated remains unknown. A proteomics analysis has identified the centrosomal kinase MARK4 as a potential USP21-interacting partner. In this work, we validate the USP21/MARK4 interaction, identifying the MARK4-binding region of USP21. Our results indicate that MARK4 interaction may regulate USP21 nucleocytoplasmic shuttling dynamics, increasing its retention in the cytoplasm. On the other hand, our data show that the centrosomal localization of both proteins is highly dynamic, and suggest that USP21 centrosomal dynamics might be modulated by MARK4.