Bereizmen-handiko fluoreszentzia-mikroskopiaren aurrerapenak organuluen mintzen arteko kontaktu-guneen azterketarako

Abstract

Membrane Contact Sites (MCS) are regions where the membranes of two adjacent organelles are positioned in close proximity. They are held together by tether proteins, and are defined by the presence of characteristic proteins and lipids. Recently, studies on MCS have become increasingly common due to the important cellular functions they perform, such as regulation of ion and phospholipid homeostasis and organelle fission and fusion processes. Additionally, an important role in human health has also been observed; in fact, they are linked to the development of various diseases, such as cancer and neurodegenerative disorders. In recent years, super-resolution fluorescence microscopy (SRFM) has emerged as a leading technique, proving to be very useful for the study of MCS. This work describes and summarizes the characteristics and biological functions of MCS and provides an in-depth analysis of the SRFM techniques that may be effective for their study. Information regarding each technique along with their advantages and limitations, has been synthesized. Finally, we have generated a gide to facilitate the selection of the most suitable SRFM method, depending on the experimental objectives and the characteristics of the technique.